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KMID : 0357220100220030093
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Journal of Korean Society Physical Therapy
2010 Volume.22 No. 3 p.93 ~ p.98
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Temporal Expression of Interleukin-1β in the Dorsal Root Ganglion in a Rat Model of Lumbar Disc Herniation
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Kim Su-Jeong
Gu Hee-Jin
Cho Yun-Woo
Park Hea-Woon
Lee Joon-Ha
Hwang Se-Jin
Ahn Sang-Ho
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Abstract
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Purpose: To investigate temporal changes in IL-1 mRNA expression in spinal dorsal horn (DH) and dorsal root ganglion (DRG) in a rat lumbar disc herniation (LDH) model.
Methods: Autologous nucleus pulposus, harvested from the tail disc between the second and third coccygeal vertebrae (Co2-3), was implanted next to the left L5 nerve root just proximal to the DRG after partial laminectomy. IL-1 mRNA expression was investigated in DRG and DH in our LDH model. Real-time PCR assays were done using a 7500 Real Time PCR system (Applied Biosystems, USA).
Results: Expression of IL-1 in DRG and DH was observed for 30 days postoperatively. Expression of IL-1 mRNA in the ipsilateral DRG of the LDH group gradually increased from 5 to 30 days after surgery. The amount of IL-1 in the contralateral DRG peaked 10 days after surgery and then gradually decreased. However, there was no difference in IL-1 mRNA expression in spinal DH between the LDH group and the sham-operated group.
Conclusion: Long-term expression of IL-1 in the LDH model may worsen the chronic pain state. Future studies on inhibition of IL-1 expression in the LDH model will be needed to develop selective treatment strategies for patients with LDH.
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KEYWORD
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Interleukin-1β, Lumbar disc herniation, pain, Dorsal root ganglion
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